Composite

Part:BBa_K1529151:Design

Designed by: Shoko Suzuki   Group: iGEM14_Tokyo_Tech   (2014-09-15)


PT7_obcB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 669
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 669
    Illegal NotI site found at 582
    Illegal NotI site found at 687
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 650
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 669
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 669
    Illegal NgoMIV site found at 460
    Illegal NgoMIV site found at 501
    Illegal NgoMIV site found at 625
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1


Source

1

References

[1]Nakata, Paul A, and Cixin He. “Oxalic acid biosynthesis is encoded by an operon in Burkholderia glumae.” FEMS microbiology letters vol. 304,2 (2010): 177-82. doi:10.1111/j.1574-6968.2010.01895.x

[2]Oh, Juntaek et al. “Structural basis for bacterial quorum sensing-mediated oxalogenesis.” The Journal of biological chemistry vol. 289,16 (2014): 11465-11475. doi:10.1074/jbc.M113.543462